Water quality is being assessed by the microbial indicators e.g. Coliforms. There are two main types of coliform bacteria, which indicate that there may be some other disease causing microbes present along with these coliforms. Total coliform are the organisms that are productive in the soil. Fecal coliform bacteria instigate in the intestines of warm-blooded organisms (mammals). They have short life span as compared to other coliforms. The presence of coliform bacteria in ground water is a worldwide issue including Pakistan. Presence of these bacteria in drinking water indicates the contamination of water with fecal materials of warm-blooded organisms specifically human beings and animals. These bacteria affect human beings more than any other creature. Some waterborne pathogenic diseases associated with these bacteria are ear infections, dysentery, typhoid fever, viral and bacterial gastroenteritis and hepatitis A. The study will not only determine the biological (fecal and total coliform) contamination in the ground water of Islamabad but will also determine its physio-chemical parameters.